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Related post: □ (b) Human tissues S (c) Neither SUMMARY OF Proventil Hfa Inhaler Cost WORK (Use standard unreduced type. Do not exceed the apace provided.) The major objective of this project is to determine host and viral factors which influence murine resistance to rabies virus. It has been shown that resistance to intraperitoneally (i.p. )"inoculated street rabies virus (SRV) was under genetic influence. Testing of second backcross progeny has confirm- ed that two genes controlled susceptibility if the first backcross parent had been selected for resistance to SRV and both parents were of similar H-2 haplotype. If the parents were of different H-2 haplotypes, or the first backcross parent was randomly selected, Purchase Proventil Online different combinations of 1-4 segrega- ting genes appeared to be involved. This secondary influence of H-2 was not obvious in earlier studies with inbred strains and F^ hybrids. Additional genetic studies have indicated that SRV resistance genes of SJL/J and CBA/J mice were allelic (identical). Pathogenesis studies have shown that 5 days after i.p. inoculation SRV entered the spinal cord of resistant and suscep- tible mice in the thoracic area, probably along splanchnic nerves of the sympathetic nervous system. Following entry into the thoracic area, SRV as- cended to the cervical area of the cord and then the brain. Minimal concentra- tions of virus were detected in the lumbar area. There was minimal replication of SRV in spinal cord and brain of resistant SJL and CBA mice. In contrast, virus concentrations were 99% greater in CNS tissues of susceptible mice as compared to resistant mice which developed clinical disease but survived. Serum neutralizing antibody appeared earlier and the titer was 100- to 1000-fold greater in resistant SJL and CBA as compared to susceptible mice. DBA/2 mice, however, which survived after Proventil Mg onset of clinical disease, had neutralizing antibody titers similar to susceptible strains. Studies of immunoglobulin isotypes have indicated there was a higher concentration of Y^g in sera of uninfected resistant SJL as compared to susceptible A/WySn mice. Immune function defects are being introduced into resistant mice to help elucidate the reasons for susceptibility differences to SRV. PHS 6040 (Rev. 1/83) 28-7 GPO 895-100 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00261-02 LPVD PERIOD COVERED October 1, 1982 to September 30. 1983 TITLE OF PROJECT 10 days) was irrelevant as a mech- anism of resistance to infection. Furthermore in resistant mice, there was no correlation between CSF antibody titer and the clinical course of disease; mice which developed clinical signs of illness but survived (DBA/2, BALB/c) had titers similar to mice which did not develop clinical illness (SJL, CBA/ J) . In addition, antibody never was detected in the CSF of mice which had serum neutralizing antibody titers of <1:320. The future course of this project will focus on understanding the protective, if any, role of neutralizing antibody in the CSF and whether it is synthesized locally in the CNS. In addition, the importance of interferon and T-lymphocytes in the CSF will be assessed in mice strains which vary in their susceptibility to Generic Proventil SRV. 28-8 PHS 6040 (Rev. 1/83) GPO 895-100 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00073-18 LPVD PERIOD COVERED
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